E-ISSN: 2148-9505
Effects of Nitric Oxide Synthase Isoforms During Experimental Tooth Movement
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Research
VOLUME: 25 ISSUE: 3
P: 239-256
December 2012

Effects of Nitric Oxide Synthase Isoforms During Experimental Tooth Movement

Turk J Orthod 2012;25(3):239-256
DOI: 10.13076/1300-3550-25-3-239
Hasan Bülbül 1
Hüseyin Ölmez 2
Erol Akın 3
Sıla Mermut Gökçe 2
Şeniz Karaçay 4
Mehmet Kürkçü 5
1. Bursa Military Hospital, Dental Service, Bursa
2. Gülhane Military Medical Academy, Dental Sciences Center, Dept. of Orthodontics, Istanbul
3. Medicana Hospital, Dept. of Orthodontics, Istanbul
4. Gülhane Military Medical Academy, Haydarpasa Training Center, Dept. of Orthodontics, Istanbul
5. Çukurova Univ., Faculty of Dentistry, Dept. of Oral and Maxillofacial Surgery, Adana, Turkey
No information available.
No information available
Received Date: 06.12.2011
Accepted Date: 24.10.2012
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ABSTRACT

Aim:

Many systemic/local factors take part in the process of bone-remodeling. Nitric oxide (NO), an important intercellular signal modulator produced by osteoblasts, is a potent regulator of bone-remodeling. The objective of the study was to investigate the effects of different NO-isoforms on bone-remodeling during orthodontic tooth movement.

Materials and Methods:

66-male, adult Sprague-Dawley rats were randomly divided into 11 groups to determine the effects of three different NO-synthase (NOS) inhibitors. All NOS-inhibitors (iNOS-inhibitor 1400W-dihydrochloride, eNOS-inhibitor L-NIO-dihydrochloride and nNOS-inhibitor Nu)-propyl-L-arginine) were administered in three different doses (10, 30 and 100μg/20μl); the remaining two groups served as control. Mandibular first molars were moved mesially with Ni-Ti closed coil-springs in all groups.

Results:

The results were evaluated histomorphometrically and parameters of trabecular bone volume (BV/TV), trabecular bone number (Tr.N) and trabecular separation (Tr.Sep) were analyzed at the interradicular bone area of the mandibular first molars. The outcomes of the WinTAS (Trabecular Analyze System, V. 1.2.9) revealed statistically significant alterations in BV/TV, Tr.N and Tr.Sep for the eNOS groups, especially for the 30\ig L-NIO dihydrochloride group.

Conclusion:

Data suggest that eNOS could be the primary NO-isoenzyme involved in bone-remodeling during orthodontic tooth movement, and optimum dose is in a certain range.

Keywords:
eNOS, iNOS, nNOS, tooth movement,