ABSTRACT
Objective
The aim of this double-blind in vivo study was to compare the extent of corrosion on the surface of nickel-titanium (NiTi) wires in various mouthwashes.
Methods
A total of 80 patients who received orthodontic treatment with as-received 0.016x0.022 inch NiTi wires were included in the study, and they were split into 4 groups. The first group used 0.05% of (225ppm Fˉ) sodium fluoride (NaF) (Colgate Plax®) containing mouthwash, 21.6% alcohol (Listerine Cool Mint®) containing mouthwash, and 0.2% clorhexidine (CHX) (Klorhex®) containing mouthwash and the control group used drinking water with melt menthol as mouthwash. After 30 days of using mouthwash, the surfaces of NiTi wires were examined with atomic force microscopy (AFM), and surface roughness values were calculated.
Results
Mouthwashes containing fluoride, essential oils, and CHX created higher surface roughness on NiTi wires than the control group. The floride-containing mouthwash group showed less corrosion than the CHX group, whereas there was no difference between the essential oil group. AFM images show supportive data with the results of the clinical study. The results were assessed using a 95% confidence interval and a significance level p<0.05.
Conclusion
CHX, essential oil, and floride-containing mouthwashes cause corrosion of NiTi wires. Floride-containing mouthwash can be preferred over CHX mouthwash due to its lesser corrosion effect.
Main Points
• Mouthwashes with different active agents are generally prescribed to orthodontic patients according to their special needs.
• Mouthwashes cause an increase in surface rougness of NiTi archwires
• Soft bristle toothbrush and aloe vera toothpaste
INTRODUCTION
During orthodontic therapy, wires, brackets, and bands increase the areas for plaque retention and make it harder for patients to clean their teeth efficiently.1 As a result, the oral flora alters, resulting in a higher risk of gingivitis and caries.2 To prevent or treat gingival disease and tooth decay, clinicians generally prescribe mouthwashes containing different active agents in accordance with the patients’ needs. These active agents include chlorhexidine (CHX), fluoride, and essential oils. Despite their frequent use, until now, only a few in vitro studies have evaluated the effects of fluoride3-5 or CHX6 and essential oils7 on nickel-containing arch wires.
Previous in vitro studies have presented potential results and conclusions but they cannot provide certain statements because they cannot reliably mimic the oral environment. The oral environment contains a variety of substances, such as saliva and acids arising from the decomposition of food. This variable medium can alter the effects of mouthwash on nickel-titanium (NiTi) wires.
The purpose of the present study was to compare the corrosion effects of mouthwashes with different active agents, such as CHX, fluoride, and essential oil, on NiTi wires by measuring the changes in surface roughness of the wires after regular mouthwash use.
METHODS
The aim of this study was explained to the participants, and informed consent was obtained before the clinical trial (registration no.: 1067). The study was approved by the Clinical Research Ethics Committee of Yeditepe University (approval no.: 62/497, date: 26.06.2015).
Sample size was calculated using G*Power (version 3.1 Franz Foul, Universitat Kiel, Germany), and the effect size was calculated to be 0.40 with an alpha value of 0.05 and a power of 80%. A total of 80 subjects (43 females and 37 males) were included in this study. These patients were treated with fixed orthodontic treatment at the Department of Orthodontics, Yeditepe University. Orthodontic appliances consisted of an average of 4 tubes, 20 bonded brackets (Mini Master Series; American Orthodontics, Sheboygan, Wisconsin, USA), and NiTi wires (G&H® wire company, Greenwood, Indiana, USA).
One hundred and twenty patients were given nutrition forms and asked to list their daily diet for seven days before participating in the study. After one week, the dietary charts of each patient were evaluated for meal and each day. Patients with a convenient diet (non-acidic and sugary snacks and drinks) were included, while 40 patients who had acidic, sugary or starch-rich eating and drinking habits were eliminated from the study. During the study period, patients were informed to avoid acidic juice, ice tea, fizzy drinks, coffee, alcohol, chocolate, and sugary snacks.
Patients were randomly assigned to four study groups of 20 patients each. Randomization was achieved by assigning patients to different groups depending on their protocol number sequence. The first group used Colgate Plax [0.05% sodium fluoride (NaF) 225ppm F-, pH: 6.9, Colgate-Palmolive Company, ABD], the second group used Listerine Cool Mint (%21.6 alcohol, pH: 4.1, Johnson & Johnson Healthcare Products Division of McNEIL-PPC, Inc, ABD), the third group used Klorhex (0.2% clorhexidine, pH: 5.5, Drogsan), and the fourth group, serving as the control group (CG), used a placebo with water, including melt menthol. Additionally, a fifth group was formed, which consisted of unused (as-received state) archwires. Patients were instructed to use mouthwash twice a day, 20 mL for 30 seconds after brushing their teeth. Standard equipment for brushing was provided to the patients. The toothpaste used was fluoride-free and aloe vera-containing [Forever Bright® (Forever Living Products, Scottsdale, AZ; 888.440.2563)], and the toothbrush was Colgate Slim Soft (Colgate-Palmolive Company, ABD) with thin and soft bristles to minimize the risk of creating surface roughness on the arch-wire while brushing.
In the upper arch 0.016x0.022 inch NiTi archwires were ligatured with elastomeric modules to prevent galvanic or fretting corrosion. The patients were instructed to brush their teeth with the provided equipment for three days before starting the use of mouthwash. After three days, the patients began using the mouthwashes as instructed for one month. Neither the participants nor the researcher knew which mouthwash was given for use in the double-blind experiment.
After one month of mouthwash use, the areas to be evaluated were marked using a diamond drill, before retrieving the arch-wire inter-bracket spaces. The retrieved wires were first cleaned under running water to remove fats and organic debris, then dried with air spray and soft paper. The retrieved archwires were placed in self-sealed envelopes, with the name of the patient and the code number of the mouthwashes recorded.
The three-dimensional surface roughnesses (Ra) of the archwires was examined using an atomic force microscopy (AFM, XE-100, PSIA Corp. Sungnam, Korea). The AFM images were obtained in non-contact mode using a cantilever (10M-NSC15, PSIA Inc.). The scan was performed at the surface with an average scanning speed of 0.5 Hz. Images with a scan size of 10 탆 x 10 탆 were obtained and analyzed using the XEI image processing program (version 1.8.0, Park Systems Corp. Suwon, Korea). The surface roughness of each wire was calculated by measuring and taking the average of the surface roughness values of three different regions (Ra).
Statistical Analysis
The Statistical Package for the Social Sciences (SPSS Inc., Chicago Illinois, USA) for Windows 21.0 was used for data analysis. The average surface roughness values, namely the Ra values of the NiTi arch-wires, were compared using the one-way (ANOVA) test. Tukey’s test was used for two-group comparisons.
RESULTS
Figure 1 shows the AFM analysis results for five different groups in the 3D images of the NiTi arch wires. After mouthwash use, the characteristic surface topographies were obvious for each group of NiTi arch wires. Greater surface roughness was evident for the CHX, alcohol or fluoride-containing mouthwash groups compared to the control and as-received groups. There was a statistically significant difference in the average surface roughness differences among the groups (Table 1, p=0.000, p<0.05).
Tukey’s test analysis results (Table 2) showed that Ra value of fluoride-containing mouthwash group (FG) was statistically significantly different from that of the CHX-containing mouthwash group (CXG) (p=0.07), as well as the control, and as-received groups (p=0.00). Likewise, the control and as-received groups had statistically significant differences (p=0.00) compared to the alcohol-containing mouthwash group (AG). No significant difference was observed between the alcohol-containing mouthwash and the other commercial mouthwashes. Although a considerable difference in surface roughness values between AG and CXG was observed (-12.2 nm), this difference was not statistically significant (p=0.076).
Discussion
In this in vivo study, the corrosion effects of different mouthwashes on NiTi wires were evaluated on the basis of previous in vitro studies.3-7 For standardization, participants were selected based on nutritional forms supported by intraoral findings, which were expected to be free of erosion and abrasion that resembling a non-acidic oral environment.
Before using the mouthwash, our patients were instructed to have mouthwash free for 3 days, based on previous studies.8, 9 They brushed only with prescribed equipment to minimize the complexity of the intraoral environment, especially in reltion to previously used mouthwash and toothpaste ingredients, which may have contained active agents similar to those evaluated in this study. Aloe vera toothpaste was preferred because it does not contain any common active mouthwash ingredients and does not cause corrosion.10
The non-contact AFM technique was preferred for high-resolution AFM because it is non-destructive and the only mode capable of genuine atomic resolution. This technique not only allows imaging but also involves high-precision manipulation.11
The study design included 0.016 x 0.022 inch NiTi wires, which were easily stabilized during AFM measurements. Related to the evaluation of friction, it has been argued that tying a wire into the bracket creates a force and tension, making it difficult to objectively measure the tying force and maintain its constancy. These compression and tension areas may show fretting and galvanic corrosion. The reason to use a leveled arch was made to observe the effects of mouthwash corrosion while minimizing other types of corrosion.12, 13
Since the frequency of the appointments for patients with fixed orthodontic appliances varies from 4 to 6 weeks, this study opted to investigate the arch wires after 1 month of clinical exposure and mouthwash use. In addition to reflecting regular appointment intervals, several studies have demonstrated that the levels of ion release caused by corrosion reaches peak level between 7 days14 to four weeks.15, 16 Thus, the highest corrosion effects can be observed within this duration.
The present study identified a significant surface roughness increase, indicating more severe corrosion morphology on NiTi archwires during the use of all the commercial mouthwash, whereas the CG showed the lowest surface roughness value (Ra=30.47 nm). Since the CG used water as a mouthwash, the results of this group mimicked the effects of the oral environment on the NiTi arch-wires. Even if there was no statistically significant difference between the as-received and CG, a quantitative increase of 11.1 nm in the surface roughness of the arch wires was observed after a month of clinical exposure. This verifies that corrosion or a proteinaceous biofilm with a rougher surface, likely forming a calcified layer, developed on the wires,11 Other similar studies have assessed the effects of the oral environment on NiTi wires using electron or optical microscopy and/or SEM.17, 18 Ghazal et al.19 reported an increase of 25.74 nm in the surface roughness of NiTi wires after a month of clinical exposure. Unlike our results, this higher amount of surface roughness found in their study may result from the use of a different arch-wire brand, partial bonding of the dentition compared to full bonded patients in our study, the lack of standardized eating habits in their study compared to the standardized diet in the present study, and differences in oral hygiene equipment and disinfection procedures.
The comparison of the surface roughness of NiTi arch wires caused by mouthwashes revealed that CXG had higher values than FG, CG, and as-received archwires, but was not significantly different from AG. The pH of CHX-containing mouthwash (pH=5.5) was more acidic than that of fluoride-containing mouthwash (pH=6.9), yet it was less acidic than that of alcohol-containing mouthwash (pH=4.1). Although the pH values of CHX- and alcohol-containing mouthwashes had different levels of acidity, there was no statistically significant difference in surface roughness. Therefore, the greater amount of corrosion on the NiTi archwires may be attributed to the corrosiveness of CHX rather than its pH value. This finding is in accordance with studies in which CHX was used as an irrigation solution20, 21 and another study that compared different mouthwashes that cause ion release from orthodontic brackets.21 On the other hand, the study by Nik et al.22 reported that CHX did not affect the surface roughness of NiTi archwires, but their study was designed with an in vitro methodology, which did not precisely mimic the oral environment.
Titanium is an essential component of NiTi archwires, and CHX gluconate is an active ingredient in CHX-containing mouthwash. A complex interaction was observed between titanium and gluconate.23, 24 The titanium oxide (TiO2) layer, which is a protective layer formed on archwires, can be increasingly dissolved in the presence of this complex reaction.25
It has been reported that at physiological pH, CHX is a highly positively charged molecule26 with an affinity for negatively charged groups. During the formation of TiO2 layer, multiple reactions and negatively charged molecules are involved. These unexpected electrochemical interactions can create a barrier to the maturation of TiO2 layer. This prolonged or inhibited formation of the TiO2 layer may explain the greater corrosion of NiTi wires in the presence of clorhexidine.
In our study, the NiTi arch-wires in the AG and FG exhibited significantly high surface roughness values. The only study that evaluated and compared the corrosion resistance of nickel-containing orthodontic arch-wires against alcohol-containing mouthwash showed that the Cr-Ni alloy exhibited the highest corrosion resistance in alcohol-containing mouthwash compared to Hank’s solution and sodium fluoride containing mouthwash.7 This opposite finding could be due to different factors, such as differences in the composition of the archwire, in vitro methodology, examination technique, and constant temperature. The corrosion rate of different types of NiTi archwires has been shown to increase with temperature.27 Bhola et al.28 evaluated the effect of alcohol-containing mouthwash on implants and reported corrosion of Ti6Al4V alloys. Our finding are in accordance with Bhola's study25, 28 and the induction of corrosion by alcohol-containing mouthwash on NiTi archwires. Alcohol-containing mouthwash contains ingredients such as essential oils, alcohol (ethanol) and it has an acidic pH of 4.1. It has been reported that with decreasing pH, the ethanol absorption of TiO2 increases.29 These cumulative factors and possible reactions could explain the protective TiO2 degradation and corrosion observed in AG.
The corrosion observed in FG was statistically higher than that in the as-received group and lower than that in the CXG group, yet there was no significant difference compared to the AG group. Although the pH of the sodium fluoride-containing mouthwash (pH=6.9) was higher, the average corrosion observed on NiTi wires was similar to that in the AG group. These results might be attributed to the presence of fluoride ions. Earlier in vitro studies5, 30, 31 have shown that fluoride-containing mouthwashes (250 ppm) can cause corrosion on NiTi arch-wires. Because our study design was in vivo, interactions within the oral environment should be considered, as they may affect the extent of corrosion.
Carbohydrate metabolism by bacteria in the oral cavity, results in the formation of acetic acid.32 When acetic acid is formed, it reacts with fluoride to produce hydrofluoric acid (HF). Higher concentrations of fluoride ions in the oral cavity result in more HF. HF reacts with TiO2, dissolving the protective TiO2 layer by forming TiO2 fluoride or titanium fluoride.33, 34 The degradation of the protective layer results in the exposure of the underlying alloy and rapid corrosion. In our study, the TiO2 layer was most likely destroyed in this manner, as shown in the Table 3.35
Corrosion causes pit defects on archwires, which create suitable areas for plaque accumulation. Since a rougher surface results in more friction between brackets and wires, we believe that the success of orthodontic treatment could be negatively affected.
Study Limitations
A limitation of the present study was the standardization of the oral environment, which is complex and contains variables. In future studies, careful consideration should be given to standardizing solid variables that affect the amount of corrosion, such as crowding, wire type, ligation technique, and oral hygiene practices.
CONCLUSION
All mouthwashes containing different active agents created corrosion on the surface topography of the NiTi archwires.
Floride-containing mouthwash can be preferred over CHX mouthwash due to its lesser corrosion effect.
The changes in the NiTi archwires caused by the oral environment were not statistically different from the as-received wires.